Fig. 3

Effects of abiraterone and MDV3100 on expression of mitochondrial marker proteins. a Western blotting analysis for expression of the ACO2, ATP synthetase, Tim 23, Tom 20 and frataxin. LNCaP cells were treated with MDV3100 or abiraterone as designed. β-actin served as a loading control. b The grouping column diagram present the relative expression of mitochondrial-related proteins normalized to β-actin in LNCaP cells after treatment with abiraterone or MDV3100. Error bars represent mean ± S.D. of three independent experiments and a representative result is shown above, *p < 0.05, **p < 0.01; ***p < 0.001 versus control, based on unpaired t-test. ACO2: aconitase2; ATP synth: ATP synthetase; Tim23: the intimal translocation enzyme of mitochondria; Tom20: the adventitia translocation enzyme of mitochondria