Fig. 4

Abiraterone and MDV3100 induced autophagy in LNCaP cells. a Representative micrographs of LNCaP cells transiently transfected with expression plasmids for Rosella-LC3 and treated with abiraterone and MDV3100. The green to red fluorescence ratio was measured in single cell under exposure described above. Error bars represent mean ± S.D. of ratios for n = 25 cells per condition. ***p < 0.001 versus control, based on unpaired t-test. b Western blotting show effects of abiraterone and MDV3100 on expression of endogenous LC3 lipidation. β-actin was served as a loading control. c Grouped column diagram present the ratio of LC3A/B-I to LC3A/B-II quantified after normalization with β-actin in LNCaP cells with abiraterone or MDV3100 treatment. Error bars represent mean ± S.D. of three independent experiments and a representative result is shown above, *p < 0.05 versus control, based on unpaired t-test. DAPI: 4′,6-diamidino-2-phenylindole; LC3: light chain 3