Fig. 5

PAX8 knockdown reduces the expression of αvβ3 on the plasma membrane. Primary hFTSEC and KURAMOCHI cells were transfected with scramble siRNA (siCTR) and PAX8 siRNA (siPAX8) and after 24 h were plated on coverslips and maintained in culture for 24 h. The confocal fluorescence analysis was performed for Integrin αvβ3 (red channel) and PAX8 (green channel). Hoechst (blue channel) was used to locate the nuclei (scale bar 10 μm). The images are representative of three independent experiments