Fig. 2

miR-1269 promotes human GC AGS/MKN-45 cell proliferation and inhibits apoptosis. a miR-1269 expression was examined in AGS/MKN-45 cells after miR-1269 overexpression. b miR-1269 expression was detected in AGS/MKN-45 cells after anti-miR-1269 treatment. c MTT assay revealed that miR-1269 overexpression promoted cell activity at 48 and 72 h after transfection. d MTT assay showed that anti-miR-1269 suppressed cell activity at 48 and 72 h after transfection. e Cell counting assay showed that miR-1269 overexpression increased GC cell proliferation. f Cell counting assays revealed that anti-miR-1269 suppressed GC cell growth. g Flow cytometry analysis of the cell cycle. The histogram represents the proportion of cells in the G0/G1, S and G2/M phases after miR-1269 overexpression. h The ratio of cells in the G0/G1, S and G2/M phases after anti-miR-1269 transfection. i Flow cytometry analysis of cell apoptosis. The data showed the ratios of early and late apoptosis after miR-1269 overexpression. j The data revealed the proportions of cells in early and late apoptosis after anti-miR-1269 transfection. Wilcoxon test, *p < 0.01, n = 3