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Fig. 4 | Cancer Cell International

Fig. 4

From: Expression profile analysis identifies IER3 to predict overall survival and promote lymph node metastasis in tongue cancer

Fig. 4

Knockdown of IER3 inhibits the proliferation of tongue cancer cells. a Protein expression of IER3 in tongue cancer cell lines (SCC-25 and Tca-8113) and oral squamous cell carcinoma cell lines (SCC-4 and SCC-9). b mRNA expression of IER3 in Tca-8113 and SCC-25 cells after transfection with siRNA. c Protein expression of IER3 in Tca-8113 and SCC-25 cells after transfection with siRNA. d Colony formation of Tca-8113 and SCC-25 cells with IER3 knockdown. Colony formation assay (left), quantification of colony formation assay (right). Data are presented as the mean ± SD (n = 3), *** indicates si-IER3-1 and si-IER3-2 versus control, P < 0.001. e OD value (450 nm) of Tca-8113 and SCC-25 cells after culture for 24 h, 48 h, and 72 h with IER3 knockdown

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