Fig. 1

Dlg5 is regulated by the ubiquitin proteasome system via an SCF ubiquitin ligase complex. a Western blot analysis of SMMC-7721 cells treated with DMSO or 10 μM MG132 for 4 h. b Western blot analysis of HepG-2 cells treated with DMSO or 10 μM MG132 for 4 h. c 293T cells were transfected with Flag-Con or Flag-Dlg5 for 36 h, cells were then treated with 10 μM MG132 for 4 h and subjected to western blot analysis with indicated antibodies. d Western blot analysis of 293T cells treated with indicated doses of MLN4924 for 4 h. e Western blot analysis of 293T cells transfected with DN-Cullin1, DN-Cullin2, DN-Cullin3, DN-Cullin4A or DN-Cullin4B plasmid, respectively. f Western blot analysis of SMMC-7721 cells transfected with siRNAs against control or Cullin1. g 293T cells were transfected with Flag-Con or Flag-Dlg5 for 36 h, cell lysate was subjected to immunoprecipitation by FlagM2 antibody. Immunoprecipitates were detected by western blot using indicated antibodies