Fig. 4
Failure to degrade Dlg5 significantly inhibited HCC cells proliferation. a The cell growth curves of control, Dlg5 WT or Dlg5 S730A cells for 4 days. b The cell viability of control, Dlg5 WT or Dlg5 S730A cells were measured by CCK-8 assay. Data represents mean ± SD. Results were averaged from three independent experiments. c Clone formation of control, Dlg5 WT or Dlg5 S730A cells were determined by soft agar colony formation assay. 1000 cells were seeded into each well, and cultured for another 2 weeks. Cells were stained with crystal violet and then counted. d Each nude mouse was subcutaneously injected with 5 × 106 cells stably expressing Dlg5 WT or Dlg5 S730A for 3 weeks. n = 10 for nude mice injected with Dlg5 WT or Dlg5 S730A cells, separately