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Fig. 3 | Cancer Cell International

Fig. 3

From: The long noncoding RNA PCGEM1 promotes cell proliferation, migration and invasion via targeting the miR-182/FBXW11 axis in cervical cancer

Fig. 3

PCGEM1 enhances CC cell migration and invasion, and induces EMT. a Transwell assay was performed in HeLa cells transfected with two different siRNAs targeting PCGEM1. The number of cells that migrated or invaded was counted in ten different fields. b Transwell assay was performed in SiHa cells transfected with PCGEM1. The number of cells that migrated or invaded was counted in ten different fields. c qRT-PCR analysis of the epithelial (E-cadherin and ZO-1) and mesenchymal markers (N-cadherin and vimentin) in HeLa cells transfected with control or PCGEM1 siRNAs. d qRT-PCR analysis of the epithelial (E-cadherin and ZO-1) and mesenchymal markers (N-cadherin and vimentin) in SiHa cells transfected with empty vector or PCGEM1. e Western blot analysis of the epithelial (E-cadherin and ZO-1) and mesenchymal markers (N-cadherin and vimentin) in HeLa and SiHa cells with PCGEM1 alteration, respectively. *p < 0.05, **p < 0.01

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