Fig. 7

FBXW11 is a target of PCGEM1 via miR-182. a SiHa cells were cotransfected with PCGMEM1 and miR-182 mimics. After 48 h, the FBXW11 mRNA levels were determined by qRT-PCR. b SiHa cells were cotransfected with PCGMEM1 and miR-182 mimics. After 48 h, the FBXW11 protein levels were determined by western blot. c HeLa cells were cotransfected with PCGMEM1 siRNAs and miR-182 inhibitors. After 48 h, the FBXW11 mRNA levels were determined by qRT-PCR. d HeLa cells were cotransfected with PCGMEM1 siRNAs and miR-182 inhibitors. After 48 h, the FBXW11 protein levels were determined by western blot. e Luciferase activity in SiHa cells cotransfected with PCGEM1 and miR-182 and luciferase reporters containing FBXW11 3′UTR. f Luciferase activity in HeLa cells cotransfected with PCGEM1 siRNAs and miR-182 inhibitors and luciferase reporters containing FBXW11 3′UTR. g The expression of FBXW11 mRNA in 68 pairs of CC and normal cervical tissues was determined by qRT-PCR. h Expression levels of PCGEM1 and FBXW11 mRNA were subjected to Pearson correlation analysis. *p < 0.05, **p < 0.01