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Fig. 2 | Cancer Cell International

Fig. 2

From: LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis

Fig. 2

LINC01006 serves as a sponge of miR-2682-5p in PC. a Subcellular fractionation was utilized to detect the distribution of LINC01006 in nuclear and cytoplasm. b Western blot assay was employed to confirm the combination between LINC01006 and Ago2. c MiRNAs that could bind with LINC01006 were obtained by referring to starbase. d The binding ability among LINC01006 and miRNAs was examined by RNA pull down assay. e The expression of miR-2682-5p in PC cell lines and normal human pancreatic duct epithelial cell line was detected by RT-qPCR. f RT-qPCR assays were used to detect the expression of miR-2682-5p in transfected cells. g RIP assay was carried out to verify that LINC01006 could bind with miR-2682-5p. h RT-qPCR assay analyzed the overexpression efficiency of miR-2682-5p in PANC-1 and BxPC-3 cells. i The binding site between LINC01006 and miR-2682-5p was obtained from starBase, which was then verified by luciferase reporter assay. *P < 0.05, **P < 0.01, ***P < 0.001

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