Fig. 3
From: LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis
HOXB8 is a target gene of miR-2682-5p in PC. a The Venn diagram displayed the potential target gene of miR-2682-5p by utilizing RNA22, microT and PicTar databases. b The expression of HOXB8 in PC cell lines and normal human pancreatic duct epithelial cell line was detected by RT-qPCR. c, d RT-qPCR analysis was used to detect the expression of miR-2682-5p and HOXB8 in transfected cells. e The binding relation among LINC01006, miR-2682-5p and HOXB8 was determined by RIP assay. f Bioinformatics and luciferase reporter assays were respectively employed to conjecture and verify the binding site between miR-2682-5p and HOXB8. g RT-qPCR and western blot assays were performed to detect the mRNA expression and protein level of HOXB8. h Cells were transfected with pcDNA3.1, pcDNA3.1/ LINC01006, pcDNA3.1/LINC01006+miR-2682-5p, pcDNA3.1/LINC01006+sh-HOXB8#1; HOXB8 mRNA was measured in RT-qPCR. *P < 0.05, **P < 0.01, ***P < 0.001