Fig. 1

Localization, titration and toxicity of MitoTracker Deep Red staining in A549 cells. a Localization of MitoTracker Deep Red staining (red) in parental (top) and paraclonal A549 cells (bottom) analyzed by immunofluorescence microscopy. Cells were counterstained with DAPI. b Titration of MitoTracker Deep Red staining. Flow cytometry-based analysis of signal intensity from mesenchymal paraclonal A549 cells after staining with the indicated concentrations of MitoTracker Deep Red dye. c Assessing toxicity of MitoTracker Deep Red staining by colony formation assay. Colonies were stained by crystal violet 2 weeks after treatment with MitoTracker Deep Red dye at the indicated concentrations, e.g. 0, 10, 25, 50, 100 and 200 nM