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Fig. 3 | Cancer Cell International

Fig. 3

From: KCNQ1OT1 aggravates cell proliferation and migration in bladder cancer through modulating miR-145-5p/PCBP2 axis

Fig. 3

MiR-145-5p targets PCBP2 in BC. a, b QRT-PCR analysis tested the expression of miR-145-5p and twelve mRNA in miR-145-5p mimics-transfected cells. b QRT-PCR assessed PCBP2 expression in BC tissues and matched control groups. c QRT-PCR assay detected PCBP2 expression in BC cell lines and SV-HUC-1 cell. d QRT-PCR detected PCBP2 expression after knockdown of KCNQ1OT1. e There displayed Starbase-predicted binding sites between PCBP2 and miR-145-5p. f, g Dual luciferase report and RIP assays validated the interactions among KCNQ1OT1, miR-145-5p and PCBP2 was in BC. h The transfection efficiency of sh-PCBP2 was guaranteed by qRT-PCR. i, j CCK-8 and colony formation assays assessed cell proliferation between sh-NC and sh-PCBP2 groups. k TUNEL examined cell apoptosis between sh-NC and sh-PCBP2 groups. l, m Wound healing and transwell assays measured cell migration and invasion between sh-NC and sh-PCBP2 groups. n Western blot observed protein level between sh-NC and sh-PCBP2 groups. GAPDH was internal control. *P < 0.05, **P < 0.01

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