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Fig. 3 | Cancer Cell International

Fig. 3

From: Silencing expression of PHF14 in glioblastoma promotes apoptosis, mitigates proliferation and invasiveness via Wnt signal pathway

Fig. 3

PHF14 gene silencing enhances apoptosis and inhibits migration and invasion of glioma cells in vitro. a, b Negative control and siPHF14 transfected U251 and A172 cells were stained with AnnexinV-PE and 7-AAD after cultured in serum-free medium for 7 days. Flow cytometric analysis was done afterwards for identification of live (both negative), early apoptosis (AnnexinV-PE-positive) and late apoptosis/dead cells (both positive). cf Migration and invasion assay showed weaker migration and invasion potential of PHF14-silenced cells. 25 thousand U251, U87MG and A172 cells were seeded into the upper chamber of a transwell insert (c, e) (Scale bar = 100 μm). After 24 h of incubation, cells which had adhered to the lower membrane of the inserts were fixed, stained with 1% crystal violet and counted for analysis (d, f). For invasion assays, the upper inserts were pre-coated with Matrigel (e, f). g, h 1000 per well negative control and siPHF14 transfected U87 cells were placed in ultra-low attachment (ULA) 96-well round bottom plates with 10% Matrigel solution and cultured for 7 days. The invasion area outlined by ImageJ or the longest invasion distance (represented by red solid line) (g) were measured by quantitation afterwards (h) (Scale bar = 50 μm) (*P < 0.05; **P = 0.01; ***P < 0.001; ****P < 0.0001)

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