Fig. 3From: Silencing expression of PHF14 in glioblastoma promotes apoptosis, mitigates proliferation and invasiveness via Wnt signal pathwayPHF14 gene silencing enhances apoptosis and inhibits migration and invasion of glioma cells in vitro. a, b Negative control and siPHF14 transfected U251 and A172 cells were stained with AnnexinV-PE and 7-AAD after cultured in serum-free medium for 7 days. Flow cytometric analysis was done afterwards for identification of live (both negative), early apoptosis (AnnexinV-PE-positive) and late apoptosis/dead cells (both positive). c–f Migration and invasion assay showed weaker migration and invasion potential of PHF14-silenced cells. 25 thousand U251, U87MG and A172 cells were seeded into the upper chamber of a transwell insert (c, e) (Scale bar = 100 μm). After 24 h of incubation, cells which had adhered to the lower membrane of the inserts were fixed, stained with 1% crystal violet and counted for analysis (d, f). For invasion assays, the upper inserts were pre-coated with Matrigel (e, f). g, h 1000 per well negative control and siPHF14 transfected U87 cells were placed in ultra-low attachment (ULA) 96-well round bottom plates with 10% Matrigel solution and cultured for 7 days. The invasion area outlined by ImageJ or the longest invasion distance (represented by red solid line) (g) were measured by quantitation afterwards (h) (Scale bar = 50 μm) (*P < 0.05; **P = 0.01; ***P < 0.001; ****P < 0.0001)Back to article page