Fig. 6

Differentially regulated transcriptional network in PHF14 depleted cells. a Pathway analysis proposed a PHF14-dependent regulation of Wnt-β-Catenin signaling pathway. Genes clusters were presented with red (− Log₂FC ≥ 1) or green color (− Log₂FC ≤ − 1). The color depth was correlated with the difference in gene expression. b Western blots showed that the levels of p-β-catenin and β-catenin decreased upon PHF14 knockdown. c PHF14-depleted cells showed higher tolerance to IWR-1-mediated inhibition of cell growth. U251 cells of both groups were treated with increased doses of IWR-1 for 48 h before determined by CCK-8 assay (450 nm). Results from three replicated experiment are shown, error bars represent standard deviation. IC50 value was determined by dose–response variable slope analysis using Graphpad Prism 7. d Quantitative RT-PCR indicated reduced mRNA level of several markers of EMT (epithelial–mesenchymal transition), PRO (Proliferation) and angiogenesis. e The high correlation of RNA expression between PHF14 and genes code for members of PRC2 including EED, EZH2, SUZ12 and RBBP4 was proved by correlation analysis. (*P < 0.05; **P = 0.01; ***P < 0.001; ****P < 0.0001)