Fig. 4

Enhanced CTHRC1 expression can promote VEGF expression in LUAD in vitro. a CTHRC1 protein was detected by WB in BEAS-2B, lung non-adenocarcinoma cell lines (L78 and H460) and lung adenocarcinoma cell lines (A549, GLC-82, SPC-A1, PC9, H1299 and H1975). b GFP expression after H1299 and A549 transfection with the high-expressions CTHRC1 lentivirus and the shCTHRC1 lentivirus respectively. CTHRC1 mRNA expression was analyzed by using the qRT-PCR. Scale bar: 100um. Error bars represent mean ± SD from three independent experiments (***P < 0.001). c CTHRC1 and VEGFA protein expression was analyzed by using the WB in the constructed Lv-shCTHRC1 H1299 cells and Lv-CTHRC1 A549 cells. GFP, Green fluorescent protein; qRT-PCR, quantitative real-time PCR; SD, standard deviation; CTHRC1, Collagen triple helix repeat containing 1; VEGFA, vascular endothelial growth factor A; LUAD, lung adenocarcinoma