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Fig. 4 | Cancer Cell International

Fig. 4

From: Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis

Fig. 4

HNF1A-AS1 acts as a ceRNA of miR-34b to promote the expression of TUFT1. a FISH assay verify HNF1A-AS1 subcellular localization. b RNA22 site predicts binding sites of HNF1A-AS1 and miR-34b. c Dual luciferase reporter gene assay was performed to verify the binding of HNF1A-AS1 to miR-34b. d The binding of HNF1A-AS1 to Ago2 detected by RIP assay. e RNA pull-down assay detected the enrichment of HNF1A-AS1 by miR-34b. f RT-qPCR detected the expression of miR-34b in DDP-sensitive cell line HeLa/S and DDP-resistant cell line HeLa/DDP. g Prediction of targeting relationship between miR-34b and TUFT1 in RNA22 website. h The targeting relationship between miR-34b and TUFT1 using luciferase activity assay. i Detection of TUFT1 expression in DDP-sensitive cell line HeLa/S and DDP-resistant cell line HeLa/DDP using RT-qPCR. *P < 0.05. Measurement data were depicted as mean ± standard deviation, and comparisons between groups were conducted by non-paired t test, and comparisons among multiple groups were assessed by one-way analysis of variance, the experiment was repeated three times

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