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Fig. 3 | Cancer Cell International

Fig. 3

From: The m6A eraser FTO facilitates proliferation and migration of human cervical cancer cells

Fig. 3

The m6A demethylase activity is required for FTO to play its oncogenic function. a Enforced FTO or FTO-mut expression in Hela cells. FTO-mut carries two point- mutations, H231A and D233A, which disrupt the enzymatic activity of FTO. GAPDH was used as a loading control; b m6A dot blot assays of Hela cells with enforced FTO or FTO-mut expression. MB, methylene blue staining (as loading control); c effects of FTO or mutant FTO overexpression on Hela cells’ growth/proliferation, ***P < 0.001. (Student’s t test); d enforced FTO or FTO-mut expression in SiHa cells. GAPDH was used as a loading control; e m6A dot blot assays of SiHa cells with enforced FTO or FTO-mut expression. MB, methylene blue staining (as loading control); f effects of FTO or mutant FTO overexpression on SiHa cells’ growth/proliferation, ***P < 0.001. (Student’s t test); g, h analysis of cell migration capacity in FTO or mutant FTO overexpressed Hela and SiHa cells

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