Fig. 3

HCG11 sponged miR-1276 to regulate CTNNB1. a, b Nuclear-cytoplasmic fractionation and FISH assay were conducted to illustrate that HCG11 was mainly in cytoplasm. c RT-qPCR assays were used to examine the expression of 19 miRNAs in GC cells. d MiR-1276, miR-483-4p and miR-875-5p expression was detected in 47 pairs of GC tissues and normal adjacent tissues. e RIP assay was applied to test the mechanical relationship of HCG11, miR-1267 and CTNNB1. f RNA pull down assay was used to demonstrate whether miR-1276 could bind to HCG11 and CTNNB1. g Luciferase reporter assay was conducted to verify competing relationship among HCG11, miR-1276 and CTNNB1. h TOP/FOP flash luciferase reporter assay was used to examine the activity of signaling decreased by sh-HCG11, which was counteracted by miR-1276 inhibitor. i Western blot assay was used to detect protein of CTNNB1 decreased by silencing HCG11, which was recovered by miR-1276 inhibitor. *P < 0.05, **P < 0.01