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Fig. 5 | Cancer Cell International

Fig. 5

From: SKA1 promotes malignant phenotype and progression of glioma via multiple signaling pathways

Fig. 5

SKA1 regulated cell cycle, EMT and Wnt/β-catenin signaling pathways in glioma cells. a Gene set enrichment analysis (GSEA) showed that there was a clear correlation between SKA1 expression and the cell cycle phase transition signature. b Expression of several cell cycle regulators and markers including FOXM1, CCNI, CCND1 and CDK1 was assessed with Western blot in U87 and U251 glioma cells with indicated treatments. c Expression of EMT-related and invasion-associated proteins including N-Cadherin, E-Cadherin, PLOD2, MMP2, Snail and Claudin were assessed with Western blot in U87 and U251 glioma cells with indicated treatments. d GSEA revealed SKA1 expression was associated with the Wnt-β-catenin signaling signature. e Expression of β-catenin and canonical Wnt/β-catenin signaling targets including CD44, c-Jun and c-Myc was examined with Western blot in U87 and U251 glioma cells with indicated treatments. All values were represented as mean ± SD of triplicate experiments (*P < 0.05)

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