Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: HAND2-AS1 inhibits invasion and metastasis of cervical cancer cells via microRNA-330-5p-mediated LDOC1

Fig. 2

Overexpression of HAND2-AS1 slows down cervical cancer cell proliferation and metastasis. a oe-HAND2-AS1 and oe-NC transfection efficiency in HeLa cells tested by RT-qPCR. b HeLa cell viability detected by CCK-8 assay. c HeLa cell invasion and migration detected by Transwell assay. d The expression of proliferation-related proteins Ki-67, PCNA, migration-related proteins N-cad, E-cad and invasion-related proteins MMP-2 and MMP-9 in HeLa cells measured by Western blot analysis. *p < 0.05 vs. the cells treated with oe-NC. All measurements were expressed by mean ± standard deviation. Unpaired t-test was used for comparison between two groups. The experiment was repeated three times. CCK-8 cell counting kit 8, NC negative control, HeLa human cervical adenocarcinoma, PCNA proliferating cell nuclear antigen, MMP-2 matrix metalloproteinases 2, MMP-9 matrix metalloproteinases 9, N-cad N-cadherin, E-cad E-cadherin

Back to article page