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Fig. 4 | Cancer Cell International

Fig. 4

From: HAND2-AS1 inhibits invasion and metastasis of cervical cancer cells via microRNA-330-5p-mediated LDOC1

Fig. 4

HAND2-AS1 mediates proliferation and metastasis of cervical cancer cells through miR-330-5p. a Transfection efficiency in HeLa cells tested by RT-qPCR. b HeLa cell viability detected by CCK-8 assay. c HeLa cell invasion and migration detected by Transwell assay. d The expression of proliferation-related proteins Ki-67, PCNA, migration-related proteins N-cad, E-cad and invasion-related proteins MMP-2 and MMP-9 in HeLa cells determined by Western blot analysis. *p < 0.05 vs. cells treated with NC mimic; #p < 0.05 vs. cells transfected with NC inhibitor. e Transfection efficiency tested by RT-qPCR. f HeLa cell viability detected by CCK-8 assay. g HeLa cell invasion and migration detected by Transwell assay. h The expression of proliferation-related proteins Ki-67, PCNA, migration-related proteins N-cad, E-cad and invasion-related proteins MMP-2 and MMP-9 in HeLa cells determined by Western blot analysis. *p < 0.05 vs. cells treated with oe-NC and mimic NC; #p < 0.05 vs. cells transfected with NC inhibitor or both oe-HAND2-AS1 and mimic NC. The data of each group were measurement data, expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multiple groups followed by Tukey’s post hoc test. The experiment was conducted three times. PCNA, proliferating cell nuclear antigen

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