Fig. 8
HAND2-AS1 inhibits tumorigenic ability and lymph node metastasis via binding to miR-330-5p in nude mice. a Expression of HAND2-AS1 in tumors from nude mice. b Tumorigenic ability of HeLa cells detected by tumor formation assay in nude mice. c miR-330-5p and LDOC1 expression in tumors determined by RT-qPCR. d Lymph node metastasis detected by HE staining (magnification: ×400; scale bar: 25 μm). e The expression of proliferation-related proteins Ki-67, PCNA, migration-related proteins N-cad, E-cad and invasion-related proteins MMP-2 and MMP-9 in tumors measured by Western blot analysis. *p < 0.05 vs. mice treated with oe-HAND2-AS1 + NC mimic. #p < 0.05 vs. mice treated with oe-HAND2-AS1 + miR-330-5p mimic. The data were measurement data, expressed as mean ± standard deviation, N = 6. Unpaired t test was used for comparison between two groups. The experiment was performed three times. HE hematoxylin–eosin