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Fig. 6 | Cancer Cell International

Fig. 6

From: Silencing lncRNA ZFAS1 or elevated microRNA-135a represses proliferation, migration, invasion and resistance to apoptosis of osteosarcoma cells

Fig. 6

Silenced ZFAS1 increases miR-135a in MG63 cells. A ZFAS1 subcellular localization predicted by online analysis. B ZFAS1 subcellular localization verified via FISH assay. C ZFAS1 and miR-135a expression in each group of MG63 cells tested by RT-qPCR. D ZFAS1 and miR-135a binding site predicted by bioinformatics website. E The regulatory relationship between ZFAS1 and miR-135a verified by dual luciferase reporter gene assay. F The binding relationship between ZFAS1 and miR-135a verified via RNA-pull down assay. The data in the figure were all measurement data, in the form of mean ± standard deviation; a vs the sh-NC group, P < 0.05; b vs the mimic-NC group, P < 0.05; c vs the sh-ZFAS1 + inhibitor NC group, P < 0.05

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