Skip to main content

Table 1 Primer sequence

From: Silencing lncRNA ZFAS1 or elevated microRNA-135a represses proliferation, migration, invasion and resistance to apoptosis of osteosarcoma cells

Gene

Primer sequence (5′–3′)

ZFAS1

Forward: 5′-ACGTGCAGACATCTACAACCT-3′

Reverse: 5′-TACTTCCAACACCCGCAT-3′

miR-135a

Forward: 5′-CTGGTAGGTATGGCTTTTTATTC-3′

Reverse: 5′-TCAACTGGTGTGGTGGAGTC-3′

APEX1

Forward: 5′-CTCACCCAGTGGCAAATCAG-3′

Reverse: 5′-TGTCACACACTGCAGGCAA-3′

U6

Forward: 5′-CTCGCTTCGGCAGCACA-3′

Reverse: 5′-AACGCTTCACGAATTTGCGT-3′

GAPDH

Forward: 5′-TCCCATCACCATCTTCCA-3′

Reverse: 5′-CATCACGCCACAGTTTTCC-3′

  1. ZFAS1 zinc finger antisense 1, miR-135a microRNA-135a, APEX1 apurinic/apyrimidinic exonuclease 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase