Fig. 1

Uev1A is involved in the regulation of AKT signaling and promotes cell survival under serum starvation conditions in breast cancer cells. a Western blot analysis of the AKT pathway proteins and phosphorylation levels from whole-cell extract (WCE) or nuclear fraction (N) in pcDNA4.0/TO/HA(+) vector (CK) or pcDNA4-UEV1A stably transfected MDA-MB-231 tet-on (TR) cells treated with doxycycline (left panel) or in MCF7 cells (right panel). The ectopic Uev1A levels were monitored by an anti-HA antibody. b, c Growth curve of control (CK) and UEV1A overexpression MDA-MB-231-TR cells under serum-supplemented and serum-deprived conditions. Cells were treated with (b) or without (c) 10% FBS and then harvested by trypsinization at indicated time points and stained with trypan blue. Viable cells were counted using a hematocytometer and an inverted microscope. Each sample was measured in triplicate and repeated 2 times. d, e Growth curve of control (CK) and UEV1A overexpression MCF7 cells under serum-supplemented and serum-deprived conditions. Experimental conditions were as described in b, c. **P < 0.01