Fig. 4

BIM is a downstream effector for Uev1A to promote cell survival under serum starvation conditions in breast cancer cells. a, c pCMV6-FOXO1 was transfected into UEV1A-overexpressed MDA-MB-231-TR (a) or NCF7 (b) cells. The protein levels of ectopic FOXO1 and BIM were detected by western blot. The protein levels of ectopic Uev1A were monitored by an anti-HA antibody. b, d pcDNA4.0/TO/HA(+)-BIM was transfected into UEV1A-overexpressed MDA-MB-231-TR (b) or MCF7 (d) cells. The protein levels of ectopic BIM were monitored by western blot. Ecotopic Uev1A levels were monitored by an anti-HA antibody. e, f Growth curve of four groups of MDA-MB-231-TR (e) or MCF7 (f) cells under serum-deprived conditions. Vector only (CK), UEV1A-overexpressed (UEV1A), FOXO1- and UEV1A-overexpressed (UEV1A + FOXO1) and BIM- and UEV1A-overexpressed (UEV1A + BIM). All MDA-MB-231-TR cells were cultured with doxycycline. Each sample was measured in triplicate and repeated at least 2 times. **P < 0.01