Fig. 5
From: Uev1A promotes breast cancer cell survival and chemoresistance through the AKT-FOXO1-BIM pathway
Uev1A promotes cell survival under serum starvation conditions through AKT pathway in a Ubc13-dependent manner. a, b The protein levels and phosphorylation levels of AKT pathway proteins were detected by western blot in pcDNA4.0/TO/HA(+) vector expressing UEV1A, UEV1A-F38E (1A-F38E) or vector only (CK) stably transfected MDA-MB-231-TR cells treated with doxycycline (a) or MCF7 (b) cells. c, d Growth curve of control (CK) and UEV1A-F38E-overexpressed (1A-F38E) MDA-MB-231-TR cells under serum-supplemented (b) and serum-deprived (c) conditions. e, f Growth curve of control (CK) and UEV1A-F38E-overexpressed (1A-F38E) MCF7 cells under serum-supplemented (e) and serum-deprived (f) conditions. Each sample was measured in triplicate and repeated at least 2 times