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Fig. 6 | Cancer Cell International

Fig. 6

From: Uev1A promotes breast cancer cell survival and chemoresistance through the AKT-FOXO1-BIM pathway

Fig. 6

Uev1A promotes AKT-mediated chemoresistance in breast cancer cells. a, b Effects of UEV1 overexpression on chemoresistance of MDA-MB-231 cells. UEV1A-overexpressed or vector control MDA-MB-231-TR cells were seeded onto 6-well culture plates with doxycycline. After a 4-h exposure to various doses of chemotherapeutic agents Paclitaxel (a) or Doxorubicin (b), the cells were cultured for an additional 7 days with drug-free medium containing 10% FBS. Then cells were harvested by trypsinization and stained with trypan blue. Cell viability was assayed by cell number counting using a hematocytometer and an inverted microscope. Each sample was measured in triplicate and repeated 2 times. c, d Dependence of chemoresistance of MDA-MB-231 cells to the AKT pathway. UEV1A-overexpressed or vector control MDA-MB-231-TR cells were seeded onto 6-well culture plates with doxycycline. With 10 μM LY294002 pretreated for 12 h, cells were exposed to various doses of Paclitaxel (c) or Doxorubicin (d) which were also in the medium with 10 µM LY294002 for 4 h and then cultured for an additional 7 days with medium containing 10% FBS and 10 µM LY294002. Cell viability assay was as described in a, b. e, f Effects of UEV1 overexpression on chemoresistance of MCF7 cells. Experimental conditions were as described in a, b. g, h Dependence of chemoresistance of MCF7 cells to the AKT pathway. Experimental conditions were as described in c, d. *P < 0.05; **P < 0.01

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