Fig. 3

CAF-derived exosomes promote cisplatin resistance in OC cells. a Morphology of fibroblasts based on the observation under the microscope and the expression of specific marker proteins (α-SMA, FAP and FSP1) in CAFs as detected by immunofluorescence staining (×200). b Ultrastructure of the exosomes under transmission electron microscopy (×5000). c Particle size of the isolated exosomes as detected by nanotrace analysis. d The expression of the exosome surface marker proteins, CD63, CD81 and TSG101, as detected by Western blot analysis. e The uptake of exosomes by A2780 cells observed under the laser confocal microscope (× 200). f The expression of miR-98-5p in A2780 cells co-cultured with exosomes as detected by RT-qPCR. g OC cell proliferation after co-culture with exosomes and treatment of cisplatin as detected by CCK-8 assay. h OC colony formation ability after co-culture with exosomes and treatment of cisplatin as detected by colony formation assay. I, Statistical analysis for h. j OC cell cycle distribution after co-culture with exosomes and treatment of cisplatin as detected by flow cytometry. k Statistical analysis for j. l OC cell apoptosis after co-culture with exosomes and treatment of cisplatin as detected by flow cytometry. m Statistical analysis for l. *p < 0.05 vs. PBS; ^#p < 0.05 vs. cells without treatment of cisplatin. These data were measurement data, expressed as mean ± standard deviation. Data between two groups were compared using unpaired t-test. Data among multiple groups were compared using one-way analysis of variance and further analyzed with Tukey’s post hoc test. Comparisons among multiple groups at different time points were analyzed using repeated measures ANOVA, followed by Bonferroni post hoc test. The experiment was repeated three times