Fig. 3
From: MiR-566 mediates cell migration and invasion in colon cancer cells by direct targeting of PSKH1
miR-566 suppressed PSKH1 expression in SW480 cells. a Computational analysis suggested putative binding sites between miR-566 and 3ʹ-UTR of human PSKH1. b SW480 cells were co-transfected with miR-566 mimic and wild-type PSKH1 3ʹ-UTR (Wt-PSKH1) or mutant PSKH1 3ʹ-UTR (Mut-PSKH1). Luciferase reporter assay was used to show PSKH1 is a direct target of miR-566. c PSKH1 protein expression was determined by Western blot assay. d, e RNA immunoprecipitation assay was performed using anti-Ago2 in SW480 cells transfected with anti-NC or miR-566 inhibitor. MiR-566 and 3′-UTR of PSKH1 mRNA levels in the anti-Ago2 or anti-IgG immunoprecipitated products were measured by RT-PCR. All experiments were performed in triplicate. *p < 0.05 vs. control