Fig. 1

Effects of NVP-BEZ235 on apoptosis and autophagy. a Cell apoptosis was analyzed in various colon cancer cells using Fluorescence-activated cell sorting (FACS) technique after the treatment of 400 nM NVP-BEZ235 for 24 h. b Hoechst33342 morphological examination of apoptosis in HCT116, RKO, SW48 cells. Cells were treated with 400 μM NVP-BEZ235 and incubated for 24 h, then stained with hochest333342, visualized under a Nikon fluorescent microscope (60×). c Representative images of GFP-LC3 translocation in HCT116, RKO, SW48 cells by treated with NVP-BEZ235, GFP-LC3 puncta was visualized by fluorescence microscopy. d The expression of LC3-II were detected by western blotting in the presence or absence of NVP-BEZ235. Similar results were obtained from three independent experiments