Fig. 2
From: Blocking AMPK/ULK1-dependent autophagy promoted apoptosis and suppressed colon cancer growth
The AMPK/ULK1 axis regulated autophagy induction by NVP-BEZ235. a The expressions of both phosphorylated and total AMPK and ULK1, LC3-II were determined by western blotting. b Representative images of GFP-LC3 translocation in HCT116 cell expressing shRNAs for AMPK or ULK1 in treatment of NVP-BEZ235. c Quantification of GFP-LC3 translocation from the representative images shown in (b). d AMPK, ULK1 and LC3-II were detected in HCT116 cell following the treatment of NVP235, with AMPK and ULK1 knockdown or not