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Fig. 3 | Cancer Cell International

Fig. 3

From: Blocking AMPK/ULK1-dependent autophagy promoted apoptosis and suppressed colon cancer growth

Fig. 3

Blocking autophagy by knockdown of AMPK/ULK1 promoted apoptosis. a Cells viability was analyzed using Cell Counting Kit-8 after AMPK or ULK1 knockdown upon NVP-BEZ235 treatment for 24 h in the three colon cancer cells (HCT116, RKO, SW48) respectively. b Colony formation of shAMPK or shULK1 in the HCT116 cell, cells were treated with NVP-BEZ235, followed with crystal violet staining of attached cells at 14 days. c Hoechst33342 morphological examination of apoptosis in shAMPK or shULK in the HCT116 cells. Cells were treated with 400 μM NVP-BEZ235 and incubated for 24 h, then stained with hochest333342. d Fluorescence-activated cell sorting (FACS) assays was used to analysis cell apoptosis. Cells were treat with NVP-BEZ235 for 24 h in HCT116, RKO and SW48 with shAMPK or shULK. e Western blotting showing the expression of LC3-II and cleaved caspase3 in shAMPK or shULK colon cancer cell after 400 μM NVP-BEZ235 treatment

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