Fig. 4
From: Blocking AMPK/ULK1-dependent autophagy promoted apoptosis and suppressed colon cancer growth
The combinational treatment of NVP-BEZ235 and CQ induces apoptosis. a Cell viability was analyzed by CCK-8 at NVP-BEZ235, CQ and the combination treatment of 400 nM NVP-BEZ235 and 50 μM CQ in HCT116, RKO and SW48 cells. b Colony formation of HCT116, RKO and SW48 cells by crystal violet staining after 24 h of different drugs treatment. c Hoechst33342 morphological examination of apoptosis after combination treatment for 24 h in three colon cancer cells. d Apoptosis in HCT-116, RKO and SW48 cells were treated with either 400 nM NVP-BEZ235, 50 μM CQ or their combination therapy was detected by flow cytometry. e Western blotting showing the expression of LC3-II and cleaved caspase3 after the treatment of NVP-BEZ235, CQ or their combination therapy for 24 h