Fig. 5From: SNHG29 regulates miR-223-3p/CTNND1 axis to promote glioblastoma progression via Wnt/β-catenin signaling pathwaySNHG29 up-regulated CTNND1 expression to promote glioblastoma progression via Wnt/β-catenin signaling pathway. a The knockdown efficiency was validated by RT-qPCR. b The genes expression was researched by microarray. c The down-regulated genes were analyzed by KEGG analysis. d, e The mRNA and protein expression of cyclin D1, β-catenin and c-myc was determined by RT-qPCR and western blot assays. f TOP-FLASH assay was used to examine the effect of SNHG29 on the activity of Wnt/β-catenin signaling pathway. g, h The assessment of mRNA and protein expression of cyclin D1, β-catenin and c-myc was conducted in RT-qPCR and western blot assays. i TOP-FLASH assay was used to examine the effect of LiCl on the activity of Wnt/β-catenin signaling pathway. j–l The investigation of cell proliferation and migration was performed by CCK-8, colony formation and transwell assays. m The examination of proteins expression related to EMT process was operated in western blot. *P < 0.05, **P < 0.01. NS not significantBack to article page