Fig. 6

Sevoflurane inhibits cell proliferation and invasion in ovarian cancer by down-regulating STC1. SKOV3 and OVCAR3 cells were transfected with pcDNA3.1-STC1 or pcDNA3.1-vector and were then co-treated with sevoflurane. a After 48 h of treatment, western blot assay was performed to examine the expression of STC1. b, c After 24 h of treatment, CCK8 assay was used to examine cell viability of SKOV3 (b) and OVCAR3 (c) cells. d Cell migration and invasion were assessed by Transwell assay. e Quantitative analysis of transwell results. f The activity of MMP-9 was examined by gelatin zymography assay. The data were expressed as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 vs. the negative control group