Fig. 2

miR-517a is upregulated in melanoma tissues and cells. a miR-517a expression in melanoma samples (n = 62) and normal skin tissues (n = 40); *p < 0.05 compared with the normal skin tissues; b miR-517a expression in melanoma cells, A375, G-361, and OCM-1 and in normal skin cell, HACAT; **p < 0.05 compared with the HACAT cell; c OD value of cells treated with H₂O₂ of various concentration for 1 h, 2 h, and 4 h; *p < 0.05 compared with cells treated with 0 μmol/L H₂O₂; d miR-517a expression after treated with H₂O₂; *p < 0.05 compared with cells treated with 0 μmol/L H₂O₂; e Cell morphology after treatment with 250 μmol/L H₂O₂ under the inverted microscope (×400). All data were expressed as mean ± standard deviation; comparisons between two groups were analyzed by unpaired t-test; comparisons among multiple groups were analyzed by one-way ANOVA; the OD values at different points were analyzed by repeated measures ANOVA