Fig. 3

Silencing miR-517a leads to OS in melanoma cells. a, b Western blot analysis of Cleaved Caspase-3, phosphorylated-ERK2 and Bax/Bcl-2 in G-361 cells treated with 250 μmol/L H₂O₂; c EdU staining of G-361 cells treated with 250 μmol/L H₂O₂ (×200). d Proliferation rate of cells treated with 250 μmol/L H₂O₂. e Cell membrane potential changes of cells treated with 250 μmol/L H₂O₂ under the fluorescence microscope (×400). f, g apoptosis rate of cells treated with 250 μmol/L H₂O₂. h Levels of T-AOC, ROS, and MDA along with SOD activity in cells treated with 250 μmol/L H₂O₂. *p < 0.05 compared with cells without treatment; ^#p < 0.05 compared with cells treated with NC inhibitor. All data were expressed as mean ± standard deviation; comparisons among multiple groups were analyzed by one-way ANOVA; the experiment was repeated three times