Fig. 4

miR-517a inhibition suppressed activation of the JNK signaling pathway by targeting CDKN1C. a Binding of miR-517a to CDKN1C confirmed by dual-luciferase reporter assay; *p < 0.05 compared with cells treated with empty vector. b miR-517a expression and mRNA level of CDKN1C in cells detected using RT-qPCR. c, d Western blot analysis of CDKN1C, JNK, and p38 proteins in cells. *p < 0.05 compared with cells without treatment; ^#p < 0.05 compared with cells treated with NC inhibitor; ^&p < 0.05, compared with the cells treated with pcDNA-NC. All data were expressed as mean ± standard deviation; comparisons among multiple groups were analyzed by one-way ANOVA; the experiment was repeated three times