Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: Silencing of microRNA-517a induces oxidative stress injury in melanoma cells via inactivation of the JNK signaling pathway by upregulating CDKN1C

Fig. 5

MiR-517a inhibition enhances OS of cells via upregulation of CDKN1C and suppression of the JNK signaling pathway. a, b Western blot analysis of Cleaved Caspase-3, Bax/Bcl-2 ratio and extent of ERK2 phosphorylation in cells treated with 250 μmol/L H2O2. c EdU staining of cells treated with 250 μmol/L H2O2 (×200). d Proliferation rate of cells treated with 250 μmol/L H2O2. e Membrane potential changes of cells treated with 250 μmol/L H2O2 under a fluorescence microscope (×400). f, g Apoptosis rate of cells treated with 250 μmol/L H2O2. h Levels of T-AOC, ROS, and MDA along with SOD activity in cells treated with 250 μmol/L H2O2; *p < 0.05 compared with cells without treatment; #p < 0.05 compared with cells treated with NC inhibitor. All data were expressed as mean ± standard deviation; comparisons among multiple groups were analyzed by one-way ANOVA; the experiment was repeated three times

Back to article page