Skip to main content

Table 1 Primer sequences of related genes for RT-qPCR

From: Silencing of microRNA-517a induces oxidative stress injury in melanoma cells via inactivation of the JNK signaling pathway by upregulating CDKN1C

Gene Primer sequence
miR-517a F: 5′-CGGCGGATCGTGCATCCCTTTA-3′
R: 5′- GTGCAGGGTCCGAGGT-3′
CDKN1C F: 5′-AACGCCGAGGACCAGAACC-3′
R: 5′-GCGAAGAAATCTGCACCGTCT -3′
U6 F: 5′-CTCGCTTCGGCAGCACA-3′
R: 5′-AACGCTTCACGAATTTGCGT-3′
GAPDH F: 5′-ATGGAGAAGGCTGGGGCTC-3′
R: 5′-AAGTTGTCATGGATGACCTTG-3′
  1. RT-qPCR reverse transcription quantitative polymerase chain reaction, miR-517a microRNA-517a, CDKN1C cyclin dependent kinase inhibitor 1C, GAPDH glyceraldehyde-3-phosphate dehydrogenase, F forward, R reverse