Fig. 1

Circ-SOX4 is upregulated in LUAD and promotes cell growth in LUAD. a Heatmap displayed the dysregulated circRNAs in LUAD tissues and adjacent normal tissues. b qRT-PCR examined circ-SOX4 expression in LUAD cell lines (A549,SPC-A1 H1299 and PC-9) and normal human lung epithelial cell line (BEAS-2B). c Schematic diagram of the genomic location and splicing pattern of circ-SOX4. d The stability of circ-SOX4 and linear SOX4 in LUAD cells treated with RNase R (normalized to mock treatment) was assessed by qRT-PCR. e qRT-PCR examined the transfection efficiency of sh-circ-SOX4 in LUAD cells. f–g CCK8 and colony formation assays were carried to measure cell proliferation in sh-circ-SOX4 transfected cells. h Cell invasion was examined by transwell assay upon circ-SOX4 knockdown. Scale bar = 50 μm. i Cell migration in cells transfected sh-circ-SOX4 was evaluated by wound healing assay. Scale bar = 100 μm. **P < 0.01