Fig. 3

Circ-SOX4 sponges miR-1270. a Subcellular fraction assay was carried out to examine the distribution of circ-SOX4 in nuclear and cytoplasm of A549 and SPC-A1 cells. b RIP assay was performed to confirm that circ-SOX4 could bind with RNA-induced silencing complex (RISC). c Several miRNAs that possess the potential of binding with circ-SOX4 were predicted by circular RNA interactome database and screened out by RIP assay d The influence of circ-SOX4 deficiency on miR-1270 expression was evaluated by qRT-PCR. e The overexpression efficiency of miR-1270 was examined in LUAD cells. f The effect of miR-1270 overexpression on the luciferase activity of reporter containing circ-SOX4 sequence was assessed by luciferase reporter assay. g The predicted binding sites between miR-1270 and circ-SOX4 were depicted. h–i Luciferase reporter and RNA pull down assays were conducted to testify the binding ability between miR-1270 and circ-SOX4. *P < 0.05, **P < 0.01, ***P < 0.001