Fig. 4

MiR-1270 could bind with its downstream mRNA PLAGL2. a Venn diagram displays the overlaps of the analysis performed in this study. b The potential mRNAs that could bind with miR-1270 were searched from PITA, RNA22, miRmap and microT databases. c qRT-PCR examined the expressions of predicted mRNAs (PLAGL2, TCF12 and GLI3) in LUAD cell lines and normal human lung epithelial cell line. d–e The mRNA and protein levels of PLAGL2 in miR-1270 mimics-transfected or sh-circ-SOX4-transfected cells were evaluated by qRT-PCR and western blot assay, respectively. f The predicted binding site between miR-1270 and PLAGL2 were shown. g Luciferase reporter assay was conducted to prove the binding ability between miR-1270 and PLAGL2. h RIP assay was performed to testify that circ-SOX4, miR-1270 and PLAGL2 in RISC. **P < 0.01