Fig. 2

CDX2 over-expression up-regulates let-7b to suppress migration and invasion of breast cancer epithelial cells. a CDX2-mediated miRNAs predicted by TransmiR database; b comparison between CDX2-regulated miRNAs predicted by TransmiR database and poorly expressed miRNAs in breast cancer-relevant microarray GSE456666; c the expression of let-7b in tissues detected by RT-qPCR (n = 86); d binding relation between let-7b promoter region and CDX2 detected by ChIP; *p < 0.05 vs. IgG; e Luciferase activities of CDX2 and let-7b; *p < 0.05 vs. NC; f let-7b expression in MCF-7 cells with different treatments after 48-h of transfection; g MCF-7 cells proliferation ability determined by EdU assay after 48-h of transfection (×200); h MCF-7 cells migration ability examined by Transwell migration assay (×200); i MCF-7 cells invasion ability examined by Transwell invasion assay (×200); j CDX2, E-cadherin and Vimentin protein expression in MCF-7 cells with different treatments tested by western blot analysis after 48-h of transfection. *p < 0.05 vs. NC. The aforementioned data were measurement data and expressed as mean ± standard deviation. Paired t-test was carried out for analysis in c; differences in d–j were analyzed with unpaired t-test. The experiment was repeated 3 times