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Fig. 5 | Cancer Cell International

Fig. 5

From: TGF-β1 stimulates epithelial–mesenchymal transition and cancer-associated myoepithelial cell during the progression from in situ to invasive breast cancer

Fig. 5

TGF-β1 regulates the morphology, migration and EMT markers of MCF-7 cells. a Immunofluorescence assay to detect CD10 and CK8/18 expression in MDA-MB-231 and MCF-7 cells. b Western blotting were used to quantify the CD10,CK8/18 and CK5/6 in MDA-MB-231 and MCF-7 cells. c TGF-β1 regulates MCF-7 cellular morphology. The bars are as indicated. d Western blots show the effect of TGF-β1 on Vimentin, N-cadherin and E-cadherin in MCF-7 cells. e qRT-PCR shows the effect of TGF-β1 on the mRNA levels of E-cadherin, N-cadherin and Vimentin in MCF-7 cells. f MTT assay assessed the effect of TGF-β1 on cell proliferation in MCF-7 cells. g, h Scratch wound-healing assay assessed the effect of TGF-β1 on the migration of MCF-7 cells. All data was showed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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