Fig. 4
From: Casticin inhibits nasopharyngeal carcinoma growth by targeting phosphoinositide 3-kinase
Casticin is a selective inhibitor of PI3K. a Schematic illustration of the KINOMEscan platform and high-throughput kinase profiling technology. The phage-tagged kinase is shown in red, ‘free’ test compound in purple and immobilized ‘bait’ ligand in blue. b Kinome-wide selectivity profiling of casticin analysed through KINOMEscan. c Binding constants (Kds) were calculated with a standard dose–response curve using the Hill equation shown in this panel. d Kinome-wide selectivity profiling of casticin was analysed through KINOMEscan. The data shown are representative of one of at least three independent experiments. e Computational docking of casticin into each active pocket of 4I2Y (PI3K110α) and docking energy obtained from casticin binding the crystal structure of PI3K110α. f Invitrogen SelectScreen™ biochemical kinase activity assay with the indications for the best potential targets of casticin