Fig. 4

Circ_LARP4 works as a sponge for miR-513b-5p to modulate LARP4. a Three websites (PITA, microT and miRmap) were utilized to predict the potential miRNAs which could bind to LARP4. b Venn diagram was used to screen out the target miRNA which could bind to circ_LARP4 and LARP4 simultaneously. RNA pull down assay and qRT-PCR tested the expression of candidate RNAs. c RNA immunoprecipitation (RIP) assay was utilized to verify that circ_LARP4, miR-513b-5p and LARP4 co-existed in RNA-induced-silencing-complex (RISC). d The potential binding sites between circ_LARP4 and miR-513b-5p were depicted by starBase website. e The binding relationship between circ_LARP4 and miR-513b-5p was attested by luciferase reporter assay. f The binding sites between LARP4 and miR-513b-5p were acquired from starBase website. g Luciferase reporter assay was performed to detect the binding correlation between LARP4 and miR-513b-5p. All data were showed as the mean ± SD. **P < 0.01