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Fig. 1 | Cancer Cell International

Fig. 1

From: Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells

Fig. 1

MLN8237 induced cell senescence, G2/M arrest,cell growth inhibition, and MYCN degradation in neuroblastoma cell line IMR32. a, f IMR32 cells were treated with 0, 0.5 μmol/l, 2 μmol/l and 10 μmol/l of MLN8237, respectively. Cell samples were collected at 6 h, 12 h, 24 h,and 48 h time points after MLN8237 treatment. Total proteins were extracted for western blot analysis for AURKA, pAURKA, MYCN, pMYCN(T58), and pMYCN(S62). b IMR32 cells were treated with 2 μmol/l of MLN8237, DMSO or no treatment as control. At day 3, cellular senescence was evaluated by SA-β-gal staining. Senescent cells show blue staining. Cell cycle c and apoptosis d analysis were assessed by flow cytometry. e Cell viability was assessed by MTT assay from day 1 to day 6 after 2 μmol/l of MLN8237 treatment, DMSO or no treatment as control. Each sample was analyzed by triplicates. Error bars correspond to the averages ± S.D. g IMR32 cells were treated with 2 μmol/l of MLN8237. Twenty-four hours later, cells were transfected with MYCN expression plasmid. Western blotting was used to verify MYCN expression. (upper figure). At 48 h after transfection, cellular senescence was evaluated by SA-β-gal staining (lower figure)

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